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PCR

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PCR
Name: Ovis-12S Type: PCR-RFLP System: QuaLitative (Light Cycler; TaqMan; Matsunaga)
Genetic Element: 12S rRNA (?) Ampl.Length: 193 GenomSize: 3.3 AnnTemp: 64
Taxonomy: Sheep PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 3 Organisation: BfR
Validation: NoValidationAvailable Sequence: NC_001941
Remarks: Reference Sequence: NC_001941
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Ovis-12S-f Sense gCTAC ATTTT CTACC CAAgA AAAT 24 62.7 54.0 64 33.3 784-807
Ovis-12S-r Antisense CTCTC gTgTg gTTgA TATAT gTAAA 25 61.6 52.8 68 36.0 976-952
Ovis-12S-Amplicon Amplicon gctac atttt ctacc caaga aaatt taata cgaaa gccat tatga aatta atagc caaag gagga tttag cagta aacta agaat agagt gctta gttga atcag gccat gaagc acgca cacac cgccc gtcac cctcc tcaag taaat atgat atact taaac ctatt tacat atatc aacca cacga gag 193 64 87.4 532 37.8 784-976
Sequence
NCBI-Acc: NC_001941 Sequence Description: Ovis aries mitochondrion, complete genome.
BaseCount: 5594 a 4289 c 2181 g 4552 t
DNA-Sequence: NC_001941.txt AminoAcid-Sequence: NC_001941.txt
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