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Name: Bos-pde Type: Real-Time PCR System: QuaNtitative
Genetic Element: Phosphodiesterase Ampl.Length: 154 GenomSize: AnnTemp: 60
Taxonomy: Cattle PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 4 Organisation: BfR
Validation: NoValidationAvailable Sequence: M27541
Remarks: Reference Sequence: M27541
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Bos-pde-f Sense ACACA ATgAA TTgAC TCCTA CCC 23 64.1 55.4 66 43.5 2994-3016
Bos-pde-r Antisense ggTgC gTAAT ggAgg AgTC 19 63.1 54.3 60 57.9 3147-3129
Bos-pde-probe Marker/Probe ATATg CCTgg AgTTT gTTTT TAAAT ATTTC AgC 33 71.9 63.1 86 30.3 3124-3092
Bos-pde-Amplicon Amplicon acaca atgaa ttgAC TCCTA CCCAT CATGC AGATt ctaac atcag gattt ttgct gcatt tgctt catct gtcct tttag agttt tcttT TTTTC TTAGC TGAAA TATTT AAAAA CAaac tccag gcata tcatt GACTC CTCCA TTACG CACC 154 60 87.5 420 36.4 2994-3147
NCBI-Acc: M27541 Sequence Description: Bovine cyclic GMP phosphodiesterase alpha subunit mRNA, complete cds.
BaseCount: 887 a 783 c 798 g 733 t
DNA-Sequence: M27541.txt AminoAcid-Sequence: M27541.txt
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