PCR |
| Name: |
ApisMellifera-Conv |
Type: |
Conventional PCR |
System: |
Qualitative |
| Genetic Element: |
18S rRNA |
Ampl. Length: |
124 |
Ampl. Position: |
13375-13498 |
Sequence Size [bp]: |
16343.A.mell. ligustica compl. mit.genome |
| AnnTemp [°C]: |
66-68 |
Taxonomy: |
Bee |
Temp. Time Program: |
Denaturation<br>2 min. / 95 °C <br>30 sec. / 94°C <br>Annealing<br>45 sec. / 57 °C <br>35 x<br>Elongation<br>45 sec. / 72 °C <br> / 4°C <br> |
| Device: |
Mastercycler |
Reagents: |
Components 1 10 Endconc.<br>10 x buffer 2.5 25 <br>MgCl2 1.25 12.5 <br>dNTP"s 0.25 2.5 <br>F Primer A.mellifera 0.5 5 200 nM<br>R Primer A.mellifera 0.5 5 200 nM<br>Taq polymerase 0.25 2.5 <br>H2O 17.75 177.5 <br>Summe 23 230 <br> |
nPrimers: |
2 |
Organisation: |
Biolytix |
| PCR Description: |
Conventional PCR for the detection of Apis mellifera |
Sequence: |
Apis.Mellifera_18S_rRNA |
| LOD [copy]: |
|
LOQ [copy]: |
|
Efficiency [%]: |
|
| Remarks: |
- Vortex the mastermix solution after preparation.
- Do 23 l of mastermix solution in a sterile tube for Mastercycler.
- Add 2 l of template DNA (100 or 10-1) to the tubes.
- For a negative control add 2 l of sterile H2O bidest.
- Close the tubes and spin short.
Put PCR fragments on an agarose gel (1%).
|
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