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PCR |
| Name: |
Sternorrhyncha-Conv |
Type: |
Conventional PCR |
System: |
Qualitative |
| Genetic Element: |
16S rRNA |
Ampl. Length: |
114 |
Ampl. Position: |
11731-11844 |
Sequence Size [bp]: |
15721.Schizaphis graminum mit. compl. genome |
| AnnTemp [°C]: |
66-69 |
Taxonomy: |
Greenbug |
Temp. Time Program: |
10` 95°C<br>30 sec 94°C<br>45 sec 57°C 40x<br>45 sec 72°C<br>5` 72°C<br> |
| Device: |
Mastercycler |
Reagents: |
Eingesetzte Primerkonzentration: 200 nM |
nPrimers: |
2 |
Organisation: |
Biolytix |
| PCR Description: |
Conventional PCR for the detection of Schizaphis graminum and other Sternorrhyncha species |
Sequence: |
Schizaphis.Graminum_Mitochondrion-Genome. |
| LOD [copy]: |
|
LOQ [copy]: |
|
Efficiency [%]: |
|
| Remarks: |
Ein Real Time PCR System ist für dieses Genom nicht angebracht, da es immer wieder zu mehrfachen Wiederholungen der gleichen Basenabfolge kommt. Eingesetzte Primerkonzentration: 200 nM |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
Ster-F |
Sense |
ACC Tgg CTT ACA CCg gTT Tg |
20 |
68.6 |
59.8 |
62.0 |
55.0 |
11731-11750 |
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Ster-R |
Antisense |
TTT gCg ACC TCg ATg TTg AA |
20 |
66.5 |
57.8 |
58.0 |
45.0 |
11825-11844 |
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