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PCR |
| Name: |
Cis_DXR |
Type: |
Real-Time PCR |
System: |
Quantitative |
| Genetic Element: |
1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) |
Ampl. Length: |
94 |
Ampl. Position: |
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Sequence Size [bp]: |
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| AnnTemp [°C]: |
60 |
Taxonomy: |
Rockrose |
Temp. Time Program: |
Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60 |
| Device: |
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Reagents: |
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nPrimers: |
3 |
Organisation: |
BfR |
| PCR Description: |
|
Sequence: |
AY297794 |
| LOD [copy]: |
2.5 |
LOQ [copy]: |
25 |
Efficiency [%]: |
97 |
| Remarks: |
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Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
Cis_DXR-f |
Sense |
AAT gTT CgA TgT CCT CCT gTT CTT |
24 |
67.1 (PCR: 60) |
58.4 |
68 |
41.67 |
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Cis_DXR-r |
Antisense |
gCT CAC TgT TTC CAC CCA CAA |
21 |
68.5 (PCR: 60) |
59.8 |
64 |
52.38 |
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Cis_DXR-probe |
Marker/Probe |
5Ž -FA M)- CC AAT ggA TCT CTC AAT ggC gCT gTC -( TAM RA) -3Å ½ |
26 |
72.1 (PCR: 60) |
67.2 |
80 |
53.85 |
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| NCBI-Acc: |
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Sequence Description: |
Cistus incanus subsp. creticus 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) mRNA, complete cds. |
| BaseCount: |
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| DNA-Sequence: |
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AminoAcid-Sequence: |
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