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You are here: Core Forms > Search PCR > PCR

PCR

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PCR

Name:

Cis_DXR

Type: Real-Time PCR System: Quantitative
Genetic Element: 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) Ampl. Length: 94 Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: 60 Taxonomy: Rockrose Temp. Time Program: Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60
Device: Reagents: nPrimers: 3 Organisation: BfR
PCR Description: Sequence: AY297794
LOD [copy]: 2.5 LOQ [copy]: 25 Efficiency [%]: 97
Remarks:

Food/Product

Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Primers

PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Cis_DXR-f Sense AAT gTT CgA TgT CCT CCT gTT CTT 24 67.1 (PCR: 60) 58.4 68 41.67
Cis_DXR-r Antisense gCT CAC TgT TTC CAC CCA CAA 21 68.5 (PCR: 60) 59.8 64 52.38
Cis_DXR-probe Marker/Probe 5Ž -FA M)- CC AAT ggA TCT CTC AAT ggC gCT gTC -( TAM RA) -3 26 72.1 (PCR: 60) 67.2 80 53.85

Sequence (more...)

NCBI-Acc:

AY297794

Sequence Description: Cistus incanus subsp. creticus 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) mRNA, complete cds.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:

Publications (more...)

Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

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