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PCR |
| Name: |
Olea_ole10 |
Type: |
Real-Time PCR |
System: |
Quantitative |
| Genetic Element: |
Allergen Ole e 10 |
Ampl. Length: |
96 |
Ampl. Position: |
|
Sequence Size [bp]: |
|
| AnnTemp [°C]: |
60 |
Taxonomy: |
Olive |
Temp. Time Program: |
Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60 |
| Device: |
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Reagents: |
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nPrimers: |
3 |
Organisation: |
BfR |
| PCR Description: |
|
Sequence: |
AY082335 |
| LOD [copy]: |
25 |
LOQ [copy]: |
250 |
Efficiency [%]: |
99 |
| Remarks: |
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Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
Olea_ole10-f |
Sense |
TAC CAA AGC AAG GGT CGA AAT G |
22 |
68.2 (PCR: 60) |
59.5 |
64 |
45.45 |
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Olea_ole10-r2 |
Antisense |
AGG GAT AAA ATT GTT TTA CTT ACT TGG GT |
29 |
68.0 (PCR: 60) |
59.3 |
76 |
31.03 |
|
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Olea_ole10-probe |
Marker/Probe |
(FA M)T CGA TTG CGA CTT TTC GGG CAC C(T AMR A) |
23 |
72.1 (PCR: 60) |
68.6 |
72 |
56.52 |
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| NCBI-Acc: |
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Sequence Description: |
Olea europaea allergen Ole e 10 mRNA, complete cds. |
| BaseCount: |
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| DNA-Sequence: |
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AminoAcid-Sequence: |
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