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You are here: Core Forms > Search PCR > PCR

PCR

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PCR

Name:

Tri_act

Type: Real-Time PCR System: Quantitative
Genetic Element: Actin Ampl. Length: 191 Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: 58 Taxonomy: Plants Temp. Time Program: Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60
Device: Reagents: nPrimers: 3 Organisation: BfR
PCR Description: Sequence: AY372368
LOD [copy]: 1 LOQ [copy]: 10 Efficiency [%]: 107
Remarks:

Food/Product

Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Primers

PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Tri_act-f Sense CTg gCC gTg ATC TAA CTg AAT CTT 24 67.7 (PCR: 58) 58.9 70 45.83
Tri_act-r Antisense ATC Agg AAg CTC ATA gTT TTT CTC AAT T 28 66.9 (PCR: 58) 58.2 74 32.14
Tri_act-probe Marker/Probe 5Ž -FA M - AA GTT CTT GTT CAT AAT CCA CAG CAA CAT AGG CAA -( TAM RA) -3 35 PCR: 58

Sequence (more...)

NCBI-Acc:

AY372368

Sequence Description: Trifolium pratense actin mRNA, complete cds.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:

Publications (more...)

Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

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