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You are here: Core Forms > Search PCR > PCR

PCR

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PCR

Name:

Til_nr

Type: Real-Time PCR System: Quantitative
Genetic Element: nitrate reductase Ampl. Length: 101 Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: 58 Taxonomy: Lime, Broad-leaved (Tilia platyphyllos) Temp. Time Program: Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60<br>
Device: Reagents: nPrimers: 3 Organisation: BfR
PCR Description: Sequence: AY138811
LOD [copy]: 25 LOQ [copy]: 250 Efficiency [%]: 98
Remarks:

Food/Product

Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Primers

PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Til_nr-f Sense ggg CAT CgA ACA TgA gCT TT 20 67.0 (PCR: 58) 58.3 60 50.00
Til_nr-r Antisense TTC AAC gAg TTT gCA Tgg gA 20 66.6 (PCR: 58) 57.8 58 45.00
Til_nr-probe Marker/Probe 5Ž -FA M)- AA AgA AgA TgC gCC TAC AAg ACC TgT TgC -( TAM RA) -3 29 72.1 (PCR: 58) 68.0 86 48.28

Sequence (more...)

NCBI-Acc:

AY138811

Sequence Description: Tilia platyphyllos nitrate reductase (nr1) mRNA, complete cds.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:

Publications (more...)

Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

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